Soybean Genomics and Improvement : Nine Historic Alfalfa Germplasm Sources

Medicago germplasm utilized for assessing SSR loci for genetic diversity.

* Accessions selected for further studies.

Number	Germplasm source	Cultivar name	Accession number	Origin	Abbreviation
1*	African 1	African	NSL 4142	Arizona, 1961	Af1
2*	African 2	‘Moapa’	NSL 4123	Navada, 1961	Af2
3*	Chilean	Arizona Common	NSL 4144	Arizona, 1961	Ch
4	Chilean	California Common	NSL 4159	Maryland, 1961
5*	Falcata	‘Wisfal’	PI 560333	Wisconsin, 1990	Fa
6	Falcata	‘Rhizoma’	PI 188868	Canada, 1950
7*	Wild 4x Falcata	Tetraploid ssp. falcata	PI 214218	Denmark, 1954	W4fa
8	Wild 4x Falcata	Tetraploid ssp. falcata	PI 502453	Russia, 1982
9*	Flemish	‘DuPuit’	PI 206103	France, 1953	Fl
10	Flemish	‘Flemade’	PI 206104	France, 1953
11*	Indian	Sirsa Type 9	PI 235736	India, 1956	In
12*	Ladak	‘Ladak’	NSL 4164	Maryland, 1961	La
13	Ladak	‘Ladak’	PI 452481	Canada, 1980
14*	Peruvian	Hairy Pervian	NSL 4143	Arizona, 1961	Pe
15	Peruvian	‘Mosefu’	PI 247790	Peru, 1958
16*	Turkistan	Kayseri	PI 171721	Turkey, 1948	Tu
17	Turkistan	Turkistan	PI 279958	Turkey, 1962
18*	Arabian 1	Fall Dormancy Class 10	UC-1887	California, 1998	Ar1
19*	Arabian 2	Fall Dormancy Class 11	UC-1465	California, 1998	Ar2
20*	Varia	‘Grimm’	NSL 4162	Maryland, 1972	Va
21	Varia	‘Miechowska’	PI 255178	Poland, 1959
22*	Wild 2x coerulea	Diploid ssp. coerulea	PI577548	Russia, 1962	W2co
23*	Wild 2x falcata	Diploid ssp. falcata	PI258754	Russia, 1959	W2fa
24*	M. truncatula	‘Jemalong’	PI442895	Australia, 1980	Mt

Genotypes

Dendrogram constructed through cluster analysis based on the PCR products of 61 polymorphic SSR primers for the phylogenetic relationships among the 9 historically recognized alfalfa germplasm sources as well as germplasm collected in the wild. Numbers at nodes are bootstrap value (%) that measures the reliability of the derived tree. Mt= M. truncatula.

3D scatter plot

3D scatter plot after multiple correspondence analyses of genetic distance among 16 germplasms based on diversity assessment using 78 SSR markers. Genotypes were clustered using Ward’s minimum variance analyses. Mt=M. truncatula, Ar2=Arabian 2, La=’Ladak’, In= Indian, Fl=Flemish, Pe=Peruvian, Af2=African 2, and Tu=Turkistan.