Projects

MOLECULAR APPROACHES FOR THE CHARACTERIZATION OF FOODBORNE PATHOGENS IN POULTRY Appropriated (D)   Accession Number: 421598

PRE-HARVEST INTERVENTIONS FOR APPLICATION DURING POULTRY PRODUCTION TO REDUCE FOOD-BORNE BACTERIAL PATHOGENS Appropriated (D)   Accession Number: 421599

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Differential Protein Expression between Poor and Robust Colonizing C. jejuni Isolates - (Abstract Only) - (18-Mar-13)

Expression and Characterization of Recombinant Campylobacter jejuni Chemotactic Proteins - (Abstract Only) - (21-Feb-13)

Construction, Expression, Purification and Antigenicity of Recombinant Campylobacter jejuni Flagellar Proteins Microbiological Research - (Peer Reviewed Journal)

Yeh, H., Hiett, K.L., Line, J.E., Oakley, B., Seal, B.S. 2013. Construction, Expression, Purification and Antigenicity of Recombinant Campylobacter jejuni Flagellar Proteins Microbiological Research. Microbiological Research. 168p.192-198.

BACTERIOCINS AND BACTERIOPHAGE LYTIC PROTEINS FROM RUSSIAN FEDERATION AND USA COLLABORATIONS TO CONTROL ANTIBIOTIC RESISTANT BACTERIAL PATHOGENS - (Abstract Only) - (16-Oct-12)

Construction, Expression, and Characterization of Flagellar Proteins for the Food-borne Pathogen Campylobacter jejuni - (Abstract Only) - (19-Jun-12)

Molecular Characterization of Podoviral Bacteriophages Virulent for Clostridium perfringens and Their Comparison with Members of the Picovirinae - (Peer Reviewed Journal)

Volozhantsev, N.V., Oakley, B., Morales, C., Verevkkin, V.V., Bannov, V.A., Popova, A.V., Zhilenkov, E.L., Svetoch, E.A., Garrish, J.K., Schegg, K.M., Woolsey, R., Quilici, D.R., Line, J.E., Hiett, K.L., Siragusa, G.R., Seal, B.S. 2012. Molecular characterization of podoviral bacteriophages virulent for clostridium perfringens and their comparison with members of the picovirinae. PLoS One. 7:e38283.

BACTERIOCINS AND BACTERIOPHAGE LYTIC PROTEINS AS ALTERNATIVES TO ANTIBIOTICS FROM RUSSIAN FEDERATION AND USA COLLABORATIONS - (Abstract Only) - (01-Apr-12)

Pyrosequencing-based validation of a simple cell-suspension polymerase chain reaction assay for Campylobacter... of high-processivity polymerase with novel internal amplification controls for rapid and specific detection. - (Peer Reviewed Journal)

Oakley, B., Line, J.E., Berrang, M.E., Johnson, J., Buhr, R.J., Cox Jr, N.A., Hiett, K.L., Seal, B.S. 2012. Pyrosequencing-based validation of a simple cell-suspension polymerase chain reaction assay for Campylobacter with application of high-processivity polymerase with novel internal amplification controls for rapid and specific detection. Diagnostic Microbiology and Infectious Disease. 72(2):131-138.

Application of high throughout sequencing to measure performance of commonly used selective cultivation methods for the food-borne pathogen campylobacter - (Peer Reviewed Journal)

Oakley, B., Morales, C., Hiett, K.L., Line, J.E., Seal, B.S. 2012. Application of high throughout sequencing to measure performance of commonly used selective cultivation methods for the food-borne pathogen campylobacter. FEMS Microbiology Ecology. 79(2):327-336.

Transmission and dose–response experiments for social animals: a reappraisal of the colonization biology of Campylobacter jejuni in chickens - (Peer Reviewed Journal)

Hiett, K.L., Line, J.E., Conlan, A.J., Coward, C., Van Diemen, P.M., Stevens, M.P., Jones, M.A. 2010. Transmission and dose–response experiments for social animals: a reappraisal of the colonization biology of Campylobacter jejuni in chickens. Journal of the Royal Society Interface. DOI: 10.1098/rsif.2011.0125.

Comparison of an automated Most Probable Number (MPN) technique to traditional plating methods for estimating populations of total aerobes, coliforms and E. coli associated with freshly processed broiler chickens - (Peer Reviewed Journal)

Line, J.E., Stern, N.J., Seal, B.S., Oakley, B. 2011. Comparison of an automated Most Probable Number (MPN) technique to traditional plating methods for estimating populations of total aerobes, coliforms and E. coli associated with freshly processed broiler chickens. Journal of Food Protection. 74(9):1558-1563.

Comparison of Cumulative Drip Sampling to Whole Carcass Rinses for Estimation of Campylobacter spp. and Quality Indicator Organisms from Processed Broiler Chickens - (Abstract Only)

Line, J.E., Stern, N.J., Oakley, B., Seal, B.S. 2011. Comparison of Cumulative Drip Sampling to Whole Carcass Rinses for Estimation of Campylobacter spp. and Quality Indicator Organisms from Processed Broiler Chickens. Meeting Abstract. International Association for Food Protection meeting.

Specificity and sensitivity of common cultivation conditions selective for Campylobacter spp. in the poultry microbiome as determined by 16S rRNA gene pyrosequencing - (Abstract Only)

Oakley, B., Morales, C., Line, J.E., Hiett, K.L., Seal, B.S. 2011. Specificity and sensitivity of common cultivation conditions selective for Campylobacter spp. in the poultry microbiome as determined by 16S rRNA gene pyrosequencing . Meeting Abstract. Volume: III Page 135.

Comparative media investigation and MLST characterization of Campylobacter spp. recovered from broiler fecal, carcass rinse, and exudate samples - (Abstract Only)

Seal, B.S., Hiett, K.L., Morales, C., Line, J.E., Oakley, B. 2011. Comparative media investigation and MLST characterization of Campylobacter spp. recovered from broiler fecal, carcass rinse, and exudate samples. Meeting Abstract. Volume: III Page:1340.

TEMPERATURE AFFECTS SOLE CARBON UTILIZATION PATTERNS OF CAMPYLOBACTER COLI 49941 - (Abstract Only)

Line, J.E., Hiett, K.L., Guard, J.Y., Seal, B.S. 2010. TEMPERATURE AFFECTS SOLE CARBON UTILIZATION PATTERNS OF CAMPYLOBACTER COLI 49941. Meeting Abstract.

Temperature affects sole carbon utilization patterns of Campylobacter coli 49941 - (Peer Reviewed Journal)

Line, J.E., Hiett, K.L., Guard, J.Y., Seal, B.S. 2010. Temperature affects sole carbon utilization patterns of Campylobacter coli 49941. Current Microbiology. 62:821-825.

Comparison of an automated Most Probable Number (MPN) technique to traditional plating methods for estimating populations of total aerobes, coliforms and E. coli associated with freshly processed broiler chickens - (Abstract Only)

Line, J.E., Stern, N.J. 2010. Comparison of an automated Most Probable Number (MPN) technique to traditional plating methods for estimating populations of total aerobes, coliforms and E. coli associated with freshly processed broiler chickens. International Association for Food Protection.

Detection of Campylobacter Colonies using Hyperspectral Imaging - (Peer Reviewed Journal)

Yoon, S.C., Lawrence, K.C., Line, J.E., Siragusa, G.R., Feldner, P.W., Park, B., Windham, W.R. 2010. Detection of Campylobacter Colonies using Hyperspectral Imaging. Sensing and Instrumentation for Food Quality and Safety. Vol. 4, Issue 1. pg. 35-49.

Differential carbon source utilization by Campylobacter jejuni strain 11168 in response to growth temperature variation - (Peer Reviewed Journal)

Line, J.E., Hiett, K.L., Guard, J.Y., Seal, B.S. 2010. Differential carbon source utilization by Campylobacter jejuni strain 11168 in response to growth temperature variation. Journal of Microbiological Methods. 80:198-202.

Phenotype microarray comparison of Campylobacter jejuni 11168 and Campylobacter coli 49941 - (Abstract Only)

Line, J.E., Hiett, K.L. 2009. Phenotype microarray comparison of Campylobacter jejuni 11168 and Campylobacter coli 49941. Campylobacter Helicobacter and Related Organisms International Workshop. Sept. 1-5, 2009. Niigata, Japan.

Hyperspectral Reflectance Imaging for Detecting a Foodborne Pathogen: Campylobacter - (Peer Reviewed Journal)

Yoon, S.C., Lawrence, K.C., Siragusa, G.R., Line, J.E., Park, B., Feldner, P.W. 2009. Hyperspectral Reflectance Imaging for Detecting a Foodborne Pathogen: Campylobacter. Transactions of the ASABE. 52(2): 651-662

Enmeration of Campylobacter spp., Escherichia coli, and Salmonella in broiler carcass rinses before and after simulated transport in artificial ice for 24 hours - (Peer Reviewed Journal)

Stern, N.J., Line, J.E. 2009. Enmeration of Campylobacter spp., Escherichia coli, and Salmonella in broiler carcass rinses before and after simulated transport in artificial ice for 24 hours. Journal of Food Protection. 72(5):1099-1101.

An individual bird challenge model is superior to group challenge for trials designed to determine colonization dose. - (Proceedings)

Line, J.E., Hiett, K.L., Conlan, A.K. 2008. An individual bird challenge model is superior to group challenge for trials designed to determine colonization dose.. American Society for Microbiology.

Effect of sponges and gas-charged incubators on recovery of Campylobacter from broiler rinses - (Abstract Only)

Line, J.E. 2008. Effect of sponges and gas-charged incubators on recovery of Campylobacter from broiler rinses. Poultry Science Association Meeting Abstract. Vol. 87(suppl. 1) p. 122-123.

Rainfall and tillage effects on transport of fecal bacteria and sex hormones 17ß-estradiol and testosterone from broiler litter applications to a Georgia Piedmont Ultisol - (Peer Reviewed Journal)

Jenkins, M., Truman, C.C., Siragusa, G.R., Line, J.E., Bailey, J.S., Frye, J.G., Endale, D.M., Franklin, D.H., Schomberg, H.H., Fisher, D.S., Sharpe, R.R. 2008. Rainfall and tillage effects on transport of fecal bacteria and sex hormones 17ß-estradiol and testosterone from broiler litter applications to a Georgia Piedmont Ultisol. Science of the Total Environment. 403(1-3):154-163.

Simplified Capacitance Monitoring for the Determination of Campylobacter spp. Growth Rates - (Peer Reviewed Journal)

Akins, D.E., Rule, P.L., Keener, M.R., Line, J.E., Simmons, I.M., Harrison, M.A., Hiett, K.L. 2009. Simplified Capacitance Monitoring for the Determination of Campylobacter spp. Growth Rates . Journal of Rapid Methods and Automation in Microbiology.

Comparison of Challenge Models for Determining the Colonization Dose of Campylobacter jejuni in Broiler Chicks - (Peer Reviewed Journal)

Line, J.E., Hiett, K.L. 2008. Comparison of Challenge Models for Determining the Colonization Dose of Campylobacter jejuni in Broiler Chicks. Poultry Science. 87:1700-1706.

Applications of Bacteriocins in Broiler Chickens to Reduce Carriage of Campylobacter and Salmonella - (Abstract Only)

Svetoch, E.A., Perelygin, V.V., Eruslanov, B.V., Line, J.E., Stern, N.J. 2008. Applications of Bacteriocins in Broiler Chickens to Reduce Carriage of Campylobacter and Salmonella. 21st International Committee on Food Microbiologty and Hygiene. P.108.

Isolation and Purification of Enteroicin E-760 with a Broad Antimicrobial Activity Against Gram-positive and Gram-negative Bacteria (AAC01569-06 Version 3) - (Peer Reviewed Journal)

Line, J.E., Svetoch, E.A., Eruslanov, B.V., Perelygin, V.V., Mitsevich, E.V., Mitsevich, I.P., Pokhilenko, V.D., Levchuk, V.P., Svetoch, O.E., Seal, B.S., Siragusa, G.R., Stern, N.J. 2008. Isolation and Purification of Enteroicin E-760 with a Broad Antimicrobial Activity Against Gram-positive and Gram-negative Bacteria (AAC01569-06 Version 3). Antimicrobial Agents and Chemotherapy. 52:1094-1100.

Hyperspectral Imaging for Detecting Pathogens Grown on Agar Plates - (Proceedings)

Yoon, S.C., Lawrence, K.C., Siragusa, G.R., Line, J.E., Park, B., Windham, W.R. 2007. Hyperspectral Imaging for Detecting Pathogens Grown on Agar Plates. Proceedings of SPIE 6761; 10-11 Sept. 2007, Boston MA.

Addition of sulfamethoxazole to selective media aids in the recovery of campylobacter spp. from broiler rinses - (Peer Reviewed Journal)

Line, J.E., Bailey, J.S., Berrang, M.E. 2008. Addition of sulfamethoxazole to selective media aids in the recovery of campylobacter spp. from broiler rinses. Journal of Rapid Methods and Automation in Microbiology. 16(1):2-12.

Selected pathogens of concern to industrial food processors: infectious, toxigenic, toxico-infectious, selected emerging pathogenic bacteria - (Book / Chapter)

Behling, R.G., Eifert, J., Erickson, M.C., Gurtler, J., Kornacki, J.L., Line, J.E., Radcliff, R., Ryser, E.T., Stawick, B., Yan, Z. 2010. Selected pathogens of concern to industrial food processors: infectious, toxigenic, toxico-infectious, selected emerging pathogenic bacteria. In: Kornack, J.L. Editor. Principles of Microbiological Troubleshooting in the Industrial Food Processing Environment. New York, NY: Springer. 140 p.

Effect of coprophagia on colonization of broiler chicks with Campylobacter jejuni - (Abstract Only)

Line, J.E., Hiett, K.L. 2007. Effect of coprophagia on colonization of broiler chicks with Campylobacter jejuni. Campylobacter Helicobacter and Related Organisms International Workshop.

Isolation and purification of a novel bacteriocin from Enterococcus spp. with broad spectrum inhibitory activity - (Abstract Only)

Line, J.E., Stern, N.J., Svetoch, E.A., Eruslanov, B.V., Perelygin, V.V., Mitsevich, E.V., Mitsevich, I.P., Pokhilenko, V.D., Levchuk, V.P., Svetoch, O.E., Seal, B.S. 2007. Isolation and purification of a novel bacteriocin from Enterococcus spp. with broad spectrum inhibitory activity. International Association for Food Protection.

Campylobacter in: Microbiological Troubleshooting in the Industrial Food Processing Environment - (Book / Chapter)

Line, J.E., Stawick, B. 2010. Campylobacter in: Microbiological Troubleshooting in the Industrial Food Processing Environment. Book Chapter.

Comparison of the microbial load of Campylobacter found on retail chicken breasts in California and Tennessee. - (Abstract Only)

Line, J.E. 2007. Comparison of the microbial load of Campylobacter found on retail chicken breasts in California and Tennessee.Meeting Abstract.

Potential Interventions for pre-harvest Control of Campylobacter in Poultry Production - (Abstract Only)

Line, J.E. 2006. Potential Interventions for pre-harvest Control of Campylobacter in Poultry Production. Canadian Campylobacter Conference.

Molecular Epidemiology, Gastrointestinal Ecology and Development of Antibiotic Alternative Interventions for Commensal Human Food-Borne Bacterial Pathogens in Poultry - (Abstract Only)

Seal, B.S., Siragusa, G.R., Hiett, K.L., Stern, N.J., Line, J.E. 2006. Molecular Epidemiology, Gastrointestinal Ecology and Development of Antibiotic Alternative Interventions for Commensal Human Food-Borne Bacterial Pathogens in Poultry. Genomes, Medicine and the Environment Meeting. p 34.

Molecular Epidemiology, Gastrointestinal Ecology and Development of Antibiotic Alternative Interventions for Commensal Human Food-Borne Bacterial Pathogens in Poultry - (Abstract Only)

Seal, B.S., Siragusa, G.R., Hiett, K.L., Stern, N.J., Line, J.E. 2006. Molecular Epidemiology, Gastrointestinal Ecology and Development of Antibiotic Alternative Interventions for Commensal Human Food-Borne Bacterial Pathogens in Poultry. Genomics.

Campylobacter-From Gate to Plate - (Abstract Only)

Robach, M., Bailey, J.S., Ingliss, D., Line, J.E., Berrang, M.E. 2006. Campylobacter-From Gate to Plate. International Association for Food Protection. 93(Abstract #S12):65.

EFFECT OF ON-FARM ACIDIFICATION TREATMENTS ON CAMPYLOBACTER AND SALMONELLA POPULATIONS IN COMMERCIAL BROILER HOUSES IN NORTHEAST GEORGIA - (Peer Reviewed Journal)

Line, J.E., Bailey, J.S. Effect of on-farm acidification treatments on campylobacter and salmonella populations in commercial broiler houses in northeast georgia. Poultry Science. 85:1529-1534.

BIPHASIC MICROTITER METHODS FOR CAMPYLOBACTER RECOVERY AND ENUMERATION - (Peer Reviewed Journal)

Line, J.E., Siragusa, G.R. 2006. Biphasic microtiter methods for campylobacter recovery and enumeration. Journal of Rapid Methods and Automation in Microbiology. 14:182-188.

Influence Of Relative Humidity On C. Jejuni Transmission in Broiler Chickens - (Peer Reviewed Journal)

Line, J.E. 2006. Influence Of Relative Humidity On C. Jejuni Transmission in Broiler Chickens. Poultry Science. 85:1145-1150.

COMPARISON OF ENRICHMENT VESSELS FOR RECOVERY OF CAMPYLOBACTER SPP. FROM BROILER RINSE SAMPLES - (Peer Reviewed Journal)

Line, J.E. 2006. Comparison of enrichment vessels for recovery of campylobacter spp. from broiler rinse samples. Journal of Rapid Methods and Automation in Microbiology. 14:110-117.

INFLUENCE OF RELATIVE HUMIDITY ON HORIZONTAL TRANSMISSION OF CAMPYLOBACTER JEJUNI IN BROILER CHICKENS - (Abstract Only)

Line, J.E. 2005. Influence of relative humidity on horizontal transmission of campylobacter jejuni in broiler chickens [abstract]. Campylobacter Helicobacter and Related Organisms International Workshop. p. 118.

ADDITION OF SULSAMETHOXAZOLE TO SELECTIVE MEDIA AIDS IN THE RECOVERY OF CAMPYLOBACTER JEJUNI - (Abstract Only)

Line, J.E., Bailey, J.S. 2005. Addition of sulsamethoxazole to selective media aids in the recovery of campylobacter jejuni. Campylobacter Helicobacter and Related Organisms International Workshop. 118

USE OF AUTOMATED REPETITIVE-SEQUENCE-BASED PCR FOR TYPING OF FOOD-BORNE BACTERIAL PATHOGENS WITH SPECIAL EMPHASIS ON CAMPYLOBACTER SPP. FROM AVIANS. - (Abstract Only)

Hiett, K.L., Siragusa, G.R., Stern, N.J., Line, J.E., Kuntz, R.L., Seal, B.S. 2005. Use of automated repetitive-sequence-based pcr for typing of food-borne bacterial pathogens with special emphasis on campylobacter spp. from avians.. Campylobacter Helicobacter and Related Organisms International Workshop. p. 11.

USE OF AUTOMATED REP-PCR FOR TYPING OF FOOD-BORNE BACTERIAL PATHOGENS FROM AVIAN SPECIES - (Abstract Only)

Line, J.E., Hiett, K.L., Siragusa, G.R., Stern, N.J., Seal, B.S. 2005. Use of automated repetitive-sequence-based pcr for typing of food-borne bacterial pathogens with special emphasis on campylobacter spp. from avian species [abstract] International Meeting on Microbial Epidemiological Markers. p. 25.

COMPARISON OF TWO TYPES OF PLATING MEDIA FOR DETECTION AND ENUMERATION OF CAMPYLOBACTER FROM POULTRY SAMPLES - (Peer Reviewed Journal)

Line, J.E., Berrang, M.E. 2005. Comparison of two types of plating media for detection and enumeration of campylobacter from poultry samples. International Journal of Poultry Science. 4(2): 81-84.

SEROLOGICAL METHODS AND SELECTIVE AGARS TO ENUMERATE CAMPYLOBACTER FROM BROILER CARCASSES: DATA FROM INTER- AND INTRA LABORATORY ANALYSIS - (Peer Reviewed Journal)

Siragusa, G.R., Line, J.E., Brooks, L., Hutchinson, T., Laster, J., Apple, R.O. 2004. Serological methods and selective agars to enumerate Campylobacter from broiler carcasses: Data from inter- and intra laboratory analysis. Journal of Food Protection. 67:901-907.

DEVELOPMENT OF A SELECTIVE BROTH MEDIUM FOR THE DETECTION OF INJURED CAMPYLOBACTER JEJUNI BY CAPACITANCE MONITORING - (Peer Reviewed Journal)

Line, J.E. 2003. Development of a selective broth medium for the detection of injured Campylobacter jejuni by capacitance monitoring. Journal of Food Protection. 67:901-907.

BIPHASIC MICROTITER METHOD FOR CAMPYLOBACTER RECOVERY AND ENUMERATION - (Abstract Only)

Line, J.E., Siragusa, G.R. 2003. Biphasic microtiter method for Campylobacter recovery and enumeration. International Journal of Medical Microbiology. 293:10.

CAMPYLOBACTER AND SALMONELLA POPULATIONS ASSOCIATED WITH CHICKENS RAISED ONACIDIFIED LITTER - (Peer Reviewed Journal) - (04-Apr-02)

DETECTION OF NATURALLY OCCURRING CAMPYLOBACTER IN POULTRY RINSES BY CAPACITANCE MONITORING - (Abstract Only)

Line, J.E. 2002. Detection of naturally occurring campylobacter in poultry rinses by capacitance monitoring. International Association for Food Protection.

IN OVO GENTAMICIN AND MUCOSAL STARTER CULTURE (TM) TO CONTROL SALMONELLA IN BROILER PRODUCTION - (Peer Reviewed Journal) - (02-Jun-01)

DEVELOPMENT OF A SELECTIVE DIFFERENTIAL AGAR FOR ISOLATION AND ENUMERATION OF CAMPYLOBACTER SPP. - (Peer Reviewed Journal) - (28-May-01)

COMPARISON OF METHODS FOR RECOVERY AND ENUMERATION OF CAMPYLOBACTER FROM FRESHLY PROCESSED BROILERS - (Peer Reviewed Journal) - (29-Jan-01)

PRE-HARVEST FOOD SAFETY IN THE UNITED STATES - (Proceedings) - (21-Apr-00)

PREHARVEST STRATEGIES FOR REDUCING PATHOGEN LEVELS IN ANIMAL PRODUCTION SYSTEMS - (Abstract Only) - (01-Mar-00)

EFFECT OF IN OVO GENTAMICIN ON EFFICACY OF MUCOSAL COMPETITIVE EXCLUSION TOCONTROL SALMONELLAE IN BROILER CHICKEN - (Abstract Only) - (15-Dec-99)

EFFECT OF IN OVO GENTAMICIN ON EFFICACY OF MUCOSAL COMPETITIVE EXCLUSION (TM) TO CONTROL SALMONELLA IN BROILER CHICKENS - (Abstract Only) - (01-Dec-99)

INCIDENCE OF SALMONELLA SPP., CAMPYLOBACTER JEJUNI AND CLOSTRIDIUM PERFRINGENS IN WILD BIRDS NEAR BROILER CHICKEN HOUSES - (Peer Reviewed Journal) - (15-Nov-99)

COMPARISON OF TWO TYPES OF PLATING MEDIA FOR DETECTION AND ENUMERATION OF CAMPYLOBACTER FROM POULTRY SAMPLES - (Abstract Only)

BERRANG, M.E., LINE, J.E. COMPARISON OF TWO TYPES OF PLATING MEDIA FOR DETECTION AND ENUMERATION OF CAMPYLOBACTER FROM POULTRY SAMPLES. CAMPYLOBACTER HELICOBACTER AND RELATED ORGANISMS INTERNATIONAL WORKSHOP. 1999.