1a.Objectives (from AD-416)
The objective of this cooperative research project is to advance cotton genomics by developing the resources for the genome sequence of the Upland cotton (Gossypium hirsutum) genetic standard, TM-1.
1b.Approach (from AD-416)
Large DNA-insert BAC libraries of the highly inbred genetic standard, TM-1, will be constructed and assembled into contigs to create a physical map. Minimum tiling path (MTP) BAC clones from selected contigs will be sequenced, and the assembled and annotated data will provide the complete cotton genome sequence with the gene content and distribution.
This is a new project, with the goals of developing resources for genome sequencing of the Gossypium hirsutum (Upland cotton) standard, TM-1, and sequencing selected chromosomes of this standard. The work will involve construction of large-insert Bacterial Artificial Chromosome (BAC) libraries which will be assembled into contigs to create a physical map. Clones from selected contigs will be sequenced, and the assembled data will provide genome sequence data for selected chromosomes. In FY 2008, 180 BACs of chromosomes 12 and 26 were sequenced by the cooperator and the raw data provided to parent project scientists. Sequence assembly and annotation, in progress, is a cooperative effort. The work, as it progresses, will develop essential molecular data for the accelerated discovery and localization of useful genes within the cotton genome. Work by this project will ultimately result in improved cotton genotypes benefiting the U.S. cotton industry. The ADODR monitors the cooperator's performance by frequent e-mail communications, periodic telephone conversations, and through monitoring and analysis of shared data. More intensive interactions with the cooperator occur, usually on an annual basis, via face-to-face interactions that include appropriate discussions of work progress, problems, and priorities.