Project Number: 5352-22430-001-27
Project Type: Trust

Start Date: Apr 01, 2011
End Date: Apr 30, 2013

Objective:
The objectives of this project are: 1) produce a transcriptome of codling moth heads; 2) identify and characterize neuropeptides expressed by codling moth; and 3) Clone and characterize neuropeptides and receptors from spotted winged Drosophila.

Approach:
1) Messenger RNA will be extracted from heads of codling moth larvae, pupae and adults, and then converted to complementary DNA (cDNA). 2) The sequences of cDNAs representing head mRNA transcripts will be determined using 454 sequencing technology. 3) The sequences of assembled head cDNAs will be used to identify those encoding neuropeptides, peptide hormones, and other potential protein targets for codling moth control. 4) cDNAs from SWD that encode neuropeptides and receptors involved in regulation of feeding and reproduction will be cloned and characterized.