ARS | Publication request: Isolation and purification of a novel bacteriocin from Enterococcus spp. with broad spectrum inhibitory activity

Submitted to: International Association for Food Protection
Publication Type: Abstract Only
Publication Acceptance Date: April 25, 2007
Publication Date: July 10, 2007
Citation: Line, J.E., Stern, N.J., Svetoch, E.A., Eruslanov, B.V., Perelygin, V.V., Mitsevich, E.V., Mitsevich, I.P., Pokhilenko, V.D., Levchuk, V.P., Svetoch, O.E., Seal, B.S. 2007. Isolation and purification of a novel bacteriocin from Enterococcus spp. with broad spectrum inhibitory activity. International Association for Food Protection.

Technical Abstract: Campylobacter jejuni is a Gram-negative human food-borne pathogen of primary importance. Poultry are frequently contaminated with C. jejuni during production with the majority of commercial U.S. flocks positive for the organism by the time the birds reach market age at about 6 weeks. There are currently few effective on-farm interventions for reducing colonization of poultry with C. jejuni. We report the isolation and purification of a 5,362 Da bacteriocin produced by an Entercoccus species isolated from chicken ceca with broad spectrum activity against both Gram-positive and Gram-negative bacteria. The bacteriocin, E-760, was isolated and purified by cation exchange and hydrophobic interaction chromatography. In vitro assays demonstrated that E-760 inhibits the growth of Salmonella enteritidis, S. choleraesuis, S. typhimurium, S. gallinarum, Escherichia coli O157:H7, Yersinia enterocolitica, Citrobacter freundii, Klebsiella pneumoniae, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus mirabilis, Morganella morganii, Staphylococcus aureus, S. epidermidis, Listeria monocytogenes, Campylobacter jejuni and 20 other Campylobacter spp. isolates. Administration of bacteriocin E-760 treated feed significantly (P< 0.05) reduced colonization of young broiler chicks experimentally challenged with two strains of C. jejuni by more than 8 log10 CFU. Bacteriocin E-760 also significantly (P<0.05) reduced colonization of naturally acquired Campylobacter spp. in market age broilers when administered in treated feed for four days prior to analysis. Bacteriocin E-760 could be developed as a practical on-farm intervention for reducing consumer exposure to pathogenic campylobacter.