ARS | Publication request: AERATION AND LENGTH OF EXPOSURE TO LIGHT AFFECT DEVELOPMENT OF FUSARIUM SOLANI AND FORMATION OF PROTEINS

Submitted to: Oklahoma Academy of Science Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 5, 1998
Publication Date: N/A

Technical Abstract: Fusarium solani was exposed to combinations of filtered air at 0, 0.1, 0.5, and 1.0 liters per minute (LPM) and multi-spectrum flourescent light for 0, 12, or 24 hr in Czapek-Dox broth. After incubation for 72 h, fungal dry weight and total protein weight, as well as numbers of protein bands in SDS-PAGE gels and their weights, were determined. As exposure to light increased and aeration flow-rate was higher, the fungal dry weight increased. Total protein was not affected by exposure to light (Avg. 0.0025 microgram/g), but was highest at 0 LPM (0.0052 microgram/g). There was no length of exposures to light by flow-rate of aeration interaction affecting fungal dry weight or total protein weight. Rate of aeration and exposure to light affected the number and weights of protein bands produced. The range of numbers of bands formed was 2 to 14, with the greatest number of bands being formed under 24 hr light. The lowest band weight was ca. 50 kD, and the highest was ca. 180 kD. Fungal dry weight was not correlated with total protein weight. This suggests that increases in fungal dry weights are due to other components of the fungal biomass. Although total protein weight appeared to be inhibited by aeration, these proteins are likely not important in the production of fungal dry weight which did not decrease as aeration flow-rate increased.