Submitted to: United States Japan Natural Resources Protein Panel
Publication Type: Proceedings
Publication Acceptance Date: November 1, 2004
Publication Date: December 1, 2004
Citation: Kepiro, J.L., McClung, A.M., Fjellstrom, R.G. 2004. Molecular genetic analysis of milling yield in rice using amplified fragment length polymorphism (aflp) and microsatellite (SSR) markers for QTL mapping. United States Japan Natural Resources Protein Panel. pp. 278-282.
Milling yield, defined as the percentage of whole rice kernels recovered after de-hulling rough rice and milling, is a critically important trait in rice. Milling yields vary considerably between cultivars, with low milling cultivars being rejected by rice growers. Molecular markers linked to milling yield will permit early and efficient selection for high milling yield in breeding lines. The cultivar Cypress is well-known for high and stable milling yield over a wide range of harvest moisture levels. A progeny population of 223 F11 lines derived from a Cypress x Panda (low milling yield parent) cross is being analyzed for segregation of milling yields over two years. The first year's data collection is completed and the regression model indicates that up to 35 percent of the variance in percent Whole grain/Total grain milled rice could be explained by six phenotypic traits that we report here. Sixty-eight AFLP primer combinations generating over 575 polymorphic markers have been scored in 120 lines of the Cypress x Panda population for identifying genomic regions associated with milling yield.