ARS | Publication request: Induction of micronuclei by palmitic acid and its unique radiolytic product 2-dodecylcyclobutanone

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: October 4, 2007
Publication Date: November 1, 2007
Citation: Sommers, C.H. 2007. Induction of micronuclei by palmitic acid and its unique radiolytic product 2-dodecylcyclobutanone. In: Proceedings of the United States-Japan (UJNR), 36th Annual Meeting, October 21-25, 2007, Tsukuba, Ibaraki, Japan. p. 59-62.

Technical Abstract: Palmitic acid (PA), one of the most abundant fatty acids in the human diet, can cause oxidative stress, DNA strand breakage, cellular necrosis and apoptosis in human and rodent cells in vitro. Radiolysis of PA leads to the formation of 2-dodecylcyclobutanone (2-DCB), a unique radiolytic product formed at part-per-million levels in fats. Like PA, 2-DCB can induce oxidative stress, DNA strand breaks, and necrosis in rodent and human cells in vitro. However, to date, there has been no direct comparison of 2-DCB and PA in any clastogenicity (DNA strand or chromosome breakage) assay. In order to address consumer concerns over the genotoxicity of unique radiolytic products present in ground beef and other foods, the ability of 2-DCB and PA to induce formation of Micronuclei (MN) in human TK6 lymphoblasts was examined. TK6 lymphoblasts were exposed to 12.5, 25 and 50uM of either PA or 2-DCB for 24 hrs. The number of MN per 1000 binucleated cells was induced 1.99 fold by 2-DCB and 2.70 fold by PA at the 50 uM concentration. Cytotoxicity was measured at 46.6 percent for 2-DCB, versus 56.4 percent for PA at the 50 uM concentration, PA and 2-DCB displayed similar clastogenic and cytotoxicity profiles in human TK6 lymphoblasts.